Қазақ ұлттық қыздар педагогикалық университеті / Казахский национальный женский педагогический университет

Постоянный URI для этого сообществаhttps://cris.qyzpu.kz/handle/123456789/1

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    Phytochemical composition and antioxidant, antimicrobial, and cytotoxic activities of different solvent extracts of Ajania fastigiata (C. Winkl.) Poljakov
    (Instituto Internacional de Ecologia (Brazil), 2025-01-01)
    Assem Mamurova
    ;
    G. Kairanova
    ;
    G.A. Amеrtayeva
    ;
    Aigul Yedilova
    ;
    Asylkhan A. Rakhymzhan
    This study investigates the phytochemical composition and biological activities of six solvent extracts-hexane, ethyl acetate, and hydroethanolic fractions-from the roots and aerial parts of Ajania fastigiata (C. Winkl.) Poljakov (Asteraceae), collected in the mountain regions of southeastern Kazakhstan. Phytochemical analysis revealed that the aerial ethyl acetate extract contained the highest levels of bioactive metabolites, including phenolic compounds (34.22 ± 1.98 mg GAE/g), flavonoids (2.74%), organic acids (1.91%), and coumarins (0.54%). Antioxidant potential was evaluated using DPPH and ABTS radical-scavenging assays, with values of SC50 = 133.98 ± 12.91 µg/mL (DPPH) and 209.21 ± 3.70 mg Trolox/g (ABTS). The minimum inhibitory concentration (MIC), determined by the broth microdilution method, showed strong antimicrobial activity of the aerial ethyl acetate fraction, particularly against P.aeruginosa (MIC = 9 µg/mL) and E.faecalis (MIC = 39 µg/mL). Cytotoxicity was assessed by the MTT assay on NIH-3T3, K562, and Saos cell lines. The aerial ethyl acetate fraction exhibited selective cytotoxic effects, with IC50 values of 210 µg/mL for K562 and 360 µg/mL for Saos, while showing negligible activity toward normal fibroblasts (IC50 > 2500 µg/mL). These results confirm the pharmacological potential of A.fastigiata as a promising natural source of antioxidant, antimicrobial, and anticancer agents.
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    BIOLOGICAL ACTIVITY AND ALKALOID COMPOSITION OF Chelidonium majus L. GROWING AT THREE SITES IN THE EASTERN PART OF KUNGEY ALATAU RIDGE (KAZAKHSTAN)
    (2025-04-20)
    A.B. Dostemessova
    ;
    Şükrü Canpolat
    ;
    Meruyert Sakenovna Kurmanbayeva
    ;
    Damezhan Sadykova
    ;
    Elif Yürümez Canpolat
    Chelidonium majus L. (family Papaveraceae), widely known as greater celandine, is a valuable plant used in traditional medicine. The present study was aimed to compare the antioxidant and antimicrobial activities, as well as the alkaloid composition in different parts of C. majus collected from various locations in Kungei-Alatau region of Kazakhstan. The amount of total phenolic compounds and the antioxidant capability were determined by using Folin-Ciocalteu phenol reagent, radical (DPPH and ABTS) scavenging, and metal ion reducing (PFRAP) assays. Antimicrobial activity was evaluated by agar disc diffusion technique. The alkaloid composition was characterized by GC-MS. The root samples yielded the highest antioxidant and antimicrobial activity compared to the aerial parts. The root extracts yielded highest scavenging activity in DPPH (1745.37 ± 1.9 μmol TE mL⁻¹), ABTS (917.97 ±10.43 μmol TE mL⁻¹), and PFRAP value (18.93 ± 0.14 mmol AAE mL⁻¹) as well as exhibited highest phenolic content (2617.62 ±5.53 μg GAE mL⁻¹). The root extracts also showed largest inhibition zone (33.0 ±0.82 mm) against Bacillus subtilis. A total of 26 alkaloids, including chelidonine, demethylchelerythrine, dihydro-chelerythrine, dihydrochelirubine, and dihydrosanguinarine, were identified. The results indicated that the roots of C. majus possess more biologically active component than its other plant parts.
    Цитирования (OpenAlex) 3Цитирования (OpenCitations) 2
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    Investigation of the Antimicrobial Activity of Biologically Active Substances Extracted from the Callus Cell Cultures of Rheum cordatum Losinsk and Rheum tataricum L. Plants (Family Polygonaceae) using Fourier infrared Spectroscopy to Analyze the Chemical Composition and Identify Active Compounds
    (2026-03-16)
    Madina Amangeldinova
    ;
    Nataliya O. Kudrina
    ;
    Gulzira Izmukhanovna Yernazarova
    ;
    Svetlana Kazbekovna Turasheva
    ;
    Metin Yildirim
    The increasing demand for novel antimicrobial agents has led to interest in plant-derived bioactive substances (BAS). This study focuses on the establishment of callus cultures from Rheum cordatum Losinsk. and Rheum tataricum L. under in vitro conditions and the evaluation of the antimicrobial properties of BAS synthesized in these callus cells. Callus cultures were induced from leaf and stem explants using Murashige-Skoog nutrient medium supplemented with 2,4-D (0.1mg/L) and BAP (0.01mg/L). Callus formation frequency was recorded for both species, and green plant regeneration from R. cordatum calluses was observed. Antimicrobial activity was assessed by testing callus-derived extracts against various microorganisms, including gram-positive Staphylococcus aureus 6538 and Salmonella abony NTCC 6017, gram-negative Escherichia coli NTCC 8439 and Klebsiella pneumonia 700603, and the yeast-like fungus Candida albicans R50. Callus induction was successfully achieved in both species, with formation frequencies of 60±6% for R. cordatum and 72±8% for R. tataricum. Extracts from R. tataricum callus cultures exhibited antimicrobial activity against S. aureus, E. coli, and K. pneumoniae. R. cordatum callus extracts showed moderate effects on these microorganisms. The results highlight the significant potential of callus-derived rhubarb extracts in addressing bacterial and fungal pathogens. The study demonstrates the efficacy of Murashige-Skoog medium supplemented with 2,4-D and BAP in promoting callus induction and growth in rhubarb species. Extracts from rhubarb callus tissues represent significant potential in developing new antimicrobial agents effective against a wide range of pathogens, including bacterial and fungal infections. Future studies will focus on the regeneration of plants from callus cultures and the detailed pharmacological profiling of their BAS.
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    Iodine-Based Coordination Compounds: A Strategy Toward Antibiotic Potentiation
    (Multidisciplinary Digital Publishing Institute, 2026-05-12)
    Daniil Shepilov
    ;
    Dana A. Askarova
    ;
    Anar Seisembekova
    ;
    Seitzhan Turganbay
    ;
    Ardak B. Jumagaziyeva
    Due to the increasing threat of antibiotic resistance and the emergence of new pathogenic strains, the development of effective combined therapeutic agents represents a crucial direction in the fight against infections. Within this study, several compounds were synthesized in which iodine is present in a coordination complex with antibiotics—sodium sulfadimidine and gentamicin sulfate. The physicochemical parameters of these compounds were investigated using capillary electrophoresis and UV spectroscopy, along with their cytotoxicity, antimicrobial, and antiviral activities. As a result of this work, two stable compounds, KC-246 and KC-248, were synthesized, demonstrating virus-inhibitory activity against herpes simplex virus and influenza A under extremely low cytotoxicity levels of 0.018–0.106 mg/mL. Additionally, they exhibited antimicrobial activity against representatives of the families Staphylococcaceae, Pseudomonadaceae, Enterobacteriaceae, Enterococcaceae, and yeast-like fungi. The minimum bactericidal concentrations (MBCs) ranged from 0.794 µg/mL to 0.198 µg/mL (KC-246) and from 2.093 µg/mL to 0.523 µg/mL (KC-248).
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    Development and Study of Hydrophilic Ointment Compositions with a Dextrin/Polyvinyl Alcohol/Iodine Complex (D/PVA/I)
    (Multidisciplinary Digital Publishing Institute, 2026-06-22)
    Zhassur Taganov
    ;
    Anel Azamatova
    ;
    Roza Karzhaubayeva
    ;
    Gulshat Baigaipova
    ;
    Zhanar A. Iskakbayeva
    Background: Iodine-based antimicrobial systems remain highly attractive due to their broad-spectrum activity; however, the clinical application of free iodine is limited by its instability and cytotoxicity. This study aimed to develop polyethylene glycol (PEG)-based hydrophilic ointment formulations containing a dextrin/polyvinyl alcohol/iodine complex (D/PVA/I) and to evaluate their physicochemical properties, antimicrobial activity, and cytotoxicity. Methods: Hydrophilic ointment formulations containing 2.5%, 5.0%, and 10.0% D/PVA/I were prepared using a PEG-based matrix composed of PEG 4000, PEG 400, and glycerol. Physicochemical characterization included organoleptic evaluation, pH measurement, rheological analysis, and UV–visible (Ultraviolet–visible) spectroscopy. Antimicrobial activity was assessed using agar diffusion and minimum bactericidal concentration (MBC) assays against Staphylococcus aureus, Escherichia coli, Enterococcus hirae, and Pseudomonas aeruginosa. Cytotoxicity was evaluated in Madin–Darby Canine Kidney (MDCK) cells using the MTT assay. Results: All formulations exhibited homogeneous semisolid structure and physiologically acceptable pH values (4.94–5.45). Rheological analysis demonstrated non-Newtonian pseudoplastic (shear-thinning) behavior. The flow behavior index (n) ranged from 0.03 to 0.33 according to the Ostwald–de Waele model, confirming shear-thinning characteristics, while viscosity increased with increasing D/PVA/I concentration. UV–visible spectroscopy confirmed the presence of triiodide ions (I3−), characterized by absorption maxima at approximately 287 and 350 nm, indicating preservation of active iodine species within the PEG matrix, while placebo (blank) formulation analysis confirmed the absence of corresponding absorption bands, demonstrating that the PEG-based matrix does not contribute to the characteristic spectral features. The formulations demonstrated broad-spectrum antimicrobial activity, with MBC values ranging from 0.01 to 0.02 µg/mL. Cytotoxicity studies revealed moderate toxicity of the D/PVA/I complex (CC50 = 0.82%) (50% cytotoxic concentration (CC50) and significantly lower toxicity of the PEG-based ointment base (CC50 = 18.38%). Conclusions: The developed PEG-based hydrophilic ointment formulations containing the D/PVA/I complex demonstrated favorable physicochemical characteristics, stability of iodine species, pronounced antimicrobial activity, and acceptable cytotoxicity profiles. These findings highlight the potential for the developed systems to be promising topical antimicrobial formulations.
    Цитирования (OpenAlex) 1