Қазақ ұлттық қыздар педагогикалық университеті / Казахский национальный женский педагогический университет

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    Method of use of biotechnological research (for example, biologically active substances of higher aquatic plants) in teaching Biology
    (Al-Farabi Kazakh National University, 2018-01-01)
    Gulzira Izmukhanovna Yernazarova
    ;
    Aliya Amangeldievna Ramazanova
    ;
    L Anuarova
    The article gives an opportunity for students to practice theoretical knowledge obtained by integratingbiotechnological research into methods of teaching biology in higher education institutions. With thehelp of biotechnological research, methods for conducting standard laboratory studies of pedagogical biologyin higher educational institutions are proposed. Laboratory classes are conducted efficiently, usingcomprehensive technological methods, depending on the pedagogical skills of the teacher. Based on theuse of biotechnological research, biologically active substances from high-water plants were determined.The subject of the study were aquatic plants Eichhornia crassipes, Pistia stratiotes, grown in a specialliquid medium, under the conditions of luministate. The amounts of biologically active substances fromaboveground and underground parts of aquatic plants were analyzed. Most of the biologically activeISSN 2520-2634 Journal of Educational Sciences. №2 (55). 2018 67Ерназарова Г.И. және т.б.substances are alkaloids, amino acids, anthraquinones, proteins, carbohydrates, coumarins,, polysaccharides,steroids, terpenes, phenolic acids and flavonoids. Methodical work is important in determiningthe method of detecting biologically active substances in special experimental work and analyzing theresults obtained by developing research abilities of students.Key words: Eichhornia crassipes, Pistia stratiotes aquatic plants, biologically active substances.
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    Extraction, Purification and Characterisation of four new alkaloids from the water plant Pistia stratiotes: POM Analyses and Identification of Potential Pharmacophore Sites
    (2023-07-24)
    Gulzira Izmukhanovna Yernazarova
    ;
    Aliya Amangeldievna Ramazanova
    ;
    Svetlana Kazbekovna Turasheva
    ;
    Faisal A. Almalki
    ;
    Taïbi Ben Hadda
    Currently, during the period of intensive development of scientific production, it is important to obtain phytopreparations from sources of raw materials of plant origin. The biological diversity of plants is amazing. The study of the composition of aquatic plants rich in active substances of biotechnological significance is currently an urgent problem. Biologically active substances are involved in various regenerative reactions and metabolic processes in the organism, being part of enzymes. Alkaloids are important nitrogenous compounds found in the plant and are widely used in medicine. The aim of the present study was to identify alkaloid compounds from the composition of the aquatic plant Pistia stratiotes, grown in a model experiment in laboratory conditions by highly effective liquid chromatography and determine the molecular structure by nuclear magnetic resonance. In addition, based on the molecular structure of alkaloid compounds found in the aquatic plant Pistia stratiotes, a study of the properties of pharmaceutical activity was conducted on the pharmaceutical site POM (Petra/Osiris/Molinspiration). According to the results of the study, 4 types of identified alkaloids were identified by the IR, 1H, 13C-NMR method. They: 1-Aza-10-methoxy-4-methyl-2-oxo-1,2-dihydroanthracene-9-O-b-D-glucopyranoside, 4,6-dimethoxy-8-prenyloxyfuroquinoline, 4,7-dimethoxy-6 - (3’ - methyl-3’ - methoxy-2 ' - oxy) butyl-oxifuroquinoline , 4,7-dimethoxy-6 - (3’ - methyl-2’ - oxy-3 ' - chlorine)butyloxifuroquinoline. As a result of the analysis of the identified POM alkaloids (Petra/Osiris/Molinspiration), predictions of antitumor activity were made. The presence of a (NHd+--- Od-) moiety indicates a potential antitumor pharmacophore site. The challenge is to verify our hypothesis experimentally and to confront virtual POM data and real anticancer screening.
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