Қазақ ұлттық қыздар педагогикалық университеті / Казахский национальный женский педагогический университет
Постоянный URI для этого сообществаhttps://cris.qyzpu.kz/handle/123456789/1
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- Некоторые метрики заблокированы вашиминастройками согласия
Тип записи:Публикация, A Method of Teaching Genetics Based on the Use of Genotoxic Studies(Ahmet Yesevi University, 2022-12-15); ; ; The purpose of this study is to give students the opportunity to develop their genetic knowledge, linking them with important problems of society. Interactive research-based teaching methods are widely used in genetic education. This allows you to determine the degree of interest of the student in the subject. A special place in teaching genetics is occupied by laboratory work. Laboratory work arouses interest, allowing students to engage in science using research methods. Modern students should learn how to use scientific models, calculate appropriate mathematical approaches and statistics to solve problems, as well as manage and integrate data. Given the importance of genetics for society, in this article we decided to focus on hemotoxins, an urgent problem at the present time. Having developed the Ames test, one of the test systems for determining genotoxicity, we focused on the features of teaching genetics. Laboratory experience helps students to control the influence of the surrounding world from hemotoxins, various factors on the processes of pollution, mutation, inheritance and, conducting the experiment, to understand mistakes, interpret the data obtained, draw the right conclusions. In this regard, interactive methods were used to consolidate the theoretical knowledge of students in the form of practice: cumulative brainstorming, minute reflection. - Некоторые метрики заблокированы вашиминастройками согласия
Тип записи:Публикация, In Vitro Measurement of Genotoxicity of Antimicrobial Dextrin–Polyvinyl Alcohol–Iodine Complex(Multidisciplinary Digital Publishing Institute, 2026-07-20) ;Tamara Bukeyeva; ;Ardak B. Jumagaziyeva ;Zhanar A. IskakbayevaSaltanat JumabayevaIodine-based antimicrobial materials are widely considered for biomedical applications due to their broad-spectrum antimicrobial activity; however, their potential genotoxicity requires systematic evaluation prior to clinical use. The aim of this study was to assess the genotoxic potential of a dextrin–polyvinyl alcohol–iodine (D/PVA/I-1) complex in accordance with OECD guidelines. Genotoxicity was evaluated in vitro using two complementary assays: the mammalian cell micronucleus test (OECD TG 487) in L5178Y TK+/− cells and the bacterial reverse mutation assay (Ames test, OECD TG 471) using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and Escherichia coli WP2 uvrA, both in the presence and absence of metabolic activation (S9). In the micronucleus assay, no statistically significant increase in micronucleus frequency was observed at concentrations ranging from 0.039 to 0.625 mg/mL compared with the negative control. In the Ames test, no increase in revertant colony numbers was detected in any tested bacterial strain at concentrations up to 1250 μg/plate. At higher concentrations, pronounced cytotoxic and bactericidal effects were observed without evidence of mutagenic activity. Overall, the results demonstrate that the D/PVA/I-1 complex does not exhibit genotoxic or mutagenic potential under the tested in vitro conditions, indicating a favorable genetic safety profile and supporting further preclinical evaluation for potential biomedical applications.
